植物乙烯气体监测系统——ETD
日期:2017-03-17 14:42:48

主要功能

本系统是全球检出限和灵敏度很高的乙烯监测系统,主要用于植物研究相关的乙烯气体监测,如种子发芽、植物生长发育、开花生理、植物器官衰老、基因表达、植物病原体相互作用、植物激素间相互作用、蔬果收货后保藏、植物抗逆性研究(干旱、高温、重金属)等。

其中乙烯气体检测仪 ETD-300 采用先进的激光技术(光声学原理),即样品乙烯在光声腔吸收激光后释放热使光声腔内部产生压力,随激光频率增减形成能被微型麦克风检测到的压力差,而乙烯浓度越高压力差越大,从而据声波强度差可实时快速测量乙烯气体(C2H4)绝对浓度;阀门控制箱 VC-6 完全自动化和电脑控制,接一个即可以使单个气体检测仪实现6个样品的自动切换测量,单个乙烯气体检测仪可以接一个或多个阀门控制箱;烃分解器 CAT-1 则利用铂金颗粒催化烃氧化分解为水蒸气和 CO2,为系统提供无烃干扰的样品空气。


测量参数

测量参数:乙烯浓度(ppbv)、气体流速(l/h)、背景值、模拟输入(V)

计算参数:乙烯产量(nl/h


连续流动测定(左)和积累测定(右)的乙烯监测数据图


应用领域

用于环境、医学、农业、工业、生态、生物等监测领域。特别适合植物生理、发育研究的超灵敏乙烯测量。



主要技术参数

参数

乙烯气体检测仪 ETD-300

阀门控制箱 VC-6

烃分解器 CAT-1


测量范围

0-2 ppm / 0-100 ppm(可调)

/

/


检出限

0.3 ppbv

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/


噪音(2σ) 

0.3 ppbv

/

/


精度

<1% 或 0.3 ppbv

0.2% FS

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稳定性

<1% 超过 24 小时

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/


零点漂移

+/-1 ppbv

/

/


测量时间

7-9 s

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/


响应时间

30 s (当流量为1 l/h时)

300 ms

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流量

0.25-5 l/h

0.25-5 l/h

0-30 l/h


校准

使用标准混合气,每年一次

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/


通道数量

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6(可增至 12, 18 等)

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测量模式

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连续测量,积累测量

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气体供应压力

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0.5-5 Bar

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过压阀

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在 5 Bar 时打开

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滤膜类型

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去除粒径 >7μm 的微粒

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最大稀释浓度

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100 ppm


输出浓度

/

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< 100 pptv


压力

/

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0-6 atm


活性催化剂

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/

Pt/SiO2


催化温度

/

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150–250 ℃


预热时间

30 min

/

< 10 min


尺寸

42x45x14 cm (48.3cm 3U 机架)

30x45x10 cm (48.3cm 2U机架)

33x24x14 cm (48.3 cm 3U 半机架)


工作温度/湿度

10-28 ℃ / 0-95 % RH

5-40 ℃ / 0-95 % RH

5-40 ℃ / 0-95 % RH


电源要求

90-264 VAC,47-63 Hz

90-264 VAC,47-63 Hz

90-264 VAC,47-63 Hz


功耗

<150 W

<20 W

85 W


进气接口

接外径 1/8'' 软管的快速接头

接外径 1/8'' 软管的快速接头

接外径 1/8'' 软管的快速接头


模拟输入

0-5 V

/

/


数据输出

USB,CSV 格式

USB,CSV 格式

/


显示

触摸屏

LED 指示灯

/



选购指南:

6通道监测系统组成如下:


                         

                                  乙烯气体检测仪ETD-300                  +                        阀门控制箱VC-6                            +          烃分解器CAT-1

注:系统中 3 个仪器都可以单独使用

可酌情选择单通道系统:乙烯气体检测仪 ETD-300+ 烃分解器 CAT-1。


产地:荷兰Sensor Sense    产地 SensorSense_log.jpg



应用举例

1.1 乙烯测定在高温胁迫研究中的应用举例

实验内容简介:以生长 3 周的拟南芥野生型 Col-0,突变体 NahG 和 opr3 植株为材料,研究了其高温胁迫下的乙烯释放。其中,野生型 Col-0 高温胁迫(38℃)下,电导率(电解质渗透率)、水杨酸和茉莉酸含量和乙烯释放增加;突变体 NahG 和 opr3 高温胁迫(38℃)下电导率、茉莉酸和乙烯释放也增加,但都低于野生型 Col-0,而高温胁迫后恢复阶段(水中 22℃)电导率明显高于 Col-0。研究结果表明:高温胁迫下,乙烯迅速产生,其生产受到茉莉酸和水杨酸的调控。总的来说,茉莉酸与水杨酸协同调节植物对高温胁迫的耐受,而乙烯主要加快细胞死亡;突变体 NahG 和 opr3 比野生型 Col-0 的耐热性差,细胞死亡多。

图1 高温处理下拟南芥植株的水杨酸(a)、电导率(b、c)和乙烯释放(d、e)

WT:拟南芥野生型;突变株opr3 ;突变株NahG以及培养基agar

Clarke, S.M., et al., Jasmonates act with salicyli c acid to confer basal thermotolerance in Arabidopsis thaliana. New Phytologist, 2009. 182(1): p. 175-187.


1.2 乙烯测定在营养缺乏(Mg)胁迫研究中的应用举例

实验内容简介:以生长5周的水培拟南芥 Col-0 植株为材料,研究了其缺镁胁迫下的乙烯释放。缺镁处理后乙烯生物合成酶基因(例如 At5g43450、At1g06620 和At2g25450)的表达水平明显上升,样品乙烯释放是对照组的两倍多,叶片中抗坏血酸 ASC 和谷胱甘肽 GSH 的氧化态比例增加。研究结果表明:植物应答缺镁胁迫存在一些独特的信号通路,且与植物激素有关,而乙烯在应答缺镁过程中发挥了关键作用;缺镁还同步增强了植物抗氧化酶活性。

表 1  镁元素缺乏处理第 8 天拟南芥新成熟叶片和根系的生理参数

应用举例-3.png

DHA:ASC,氧化态脱氢抗坏血酸:抗坏血酸;GSSG : GSH,氧化型谷胱甘肽:谷胱甘肽;Ctrl,镁元素充足的植株;-Mg,镁元素缺乏的植株

Hermans, C., et al., Systems analysis of the responses to long-term magnesium deficiency and restoration in Arabidopsis thaliana. New Phytologist, 2010. 187(1): p. 132-144.


1.3 乙烯测定在病菌感染研究中的应用举例

实验内容简介:以品种为 Money Maker 和 Daniela 的成熟番茄果实为材料,研究了其感染番茄灰霉病菌株 VTF1 的乙烯释放。灰霉病菌可以在体外产生乙烯,其乙烯释放与其说与分生孢子萌发相关,不如说与菌丝生长更相关,且分生孢子浓度越大真菌的乙烯释放越多。感染灰霉病的两种番茄的乙烯释放规律与灰霉病菌类似;但释放量是其 100 倍。结合受感染番茄的细胞学参数,研究结果表明:番茄-真菌系统的乙烯释放不是由番茄灰霉病菌引起的,虽说与其内部的真菌生长速率十分同步。

应用举例-4.png

图 2 真菌(160 μl 悬浮液)的乙烯产量

● 1.5*108 灰霉病菌分生孢子 ml-1  ▲ 2*107 灰霉病菌分生孢子 ml-1  ■ 2*105 灰霉病菌分生孢子 ml-1


应用举例-5.png

图3  模拟感染和不同浓度番茄灰霉病菌感染的两种番茄的乙烯释放

A.番茄品种 Money Maker;B.番茄品种 Daniela;

○ 模拟番茄灰霉病菌感染  ● 1.5*108 灰霉病菌分生孢子 ml-1  ▲ 2*107 灰霉病菌分生孢子 ml-1  ■ 2*105 灰霉病菌分生孢子 ml-1

Cristescu, S.M., et al., Ethylene Production by Botrytis cinerea In Vitro and in Tomatoes. Applied and Environmental Microbiology, 2002. 68 (11): p. 5342-5350.


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